Cell Fractionation and Mitochondrial Function Lab Report

Cell Fractionation and Mitochondrial Function - Lab Report manikinn the case of rotors which hold the provides on an angle) and, particularly in the first spin, cell membrane material may appear as a be adrift skin or pellicle on the surface of the tube. The remaining liquid in the tube is called the supernatant, or supe for short.The higher the concentration of mitochondria, the lower the absorbance density. This is because in the mitochondria cell membrane, on that point is an integral membrane protein referred to as succinate dehydrogenase that has the capacity to reduce the DCIP and when DCIP is reduced, the OD is reduced as well. Basing on the graphs represented above, it is clearly evident that the mitochondria came down in stroke from 12000xg.In graph representing tube 1, it is observed that the O.D decreased with time. This means that There was mitochondria in the solution which reduced the DCIP with time. The enzyme Succinate dehydrogenase is the one responsible for thi s reaction.In graph representing tube 4,it is observed that, there is no change of O.D with time.this means that mitochondria was absent indicating that there was no mitochondria in the supernatant. Hence went down in the pellet as indicated in graph representing tube 2.In graph representing tube 6 it is also observed that, there is no change in the O.D with time and this as well indicate that there was no mitochondria in the tube because it was water which was added. Therefore in conclusion, it is evident that the mitochondria came down in the